Generation of high-spin iron(I) in a protein environment using cryoreduction. Academic Article uri icon


  • High-spin Fe(1+) sites are potentially important in iron-sulfur proteins but are rare in synthetic compounds and unknown in metalloproteins. Here, we demonstrate a spectroscopically characterized example of high-spin non-heme Fe(1+) in a protein environment. Cryoreduction of Fe(2+)-substituted azurin at 77 K with (60)Co radiation generates a new species with a S = (3)/2 (high-spin) Fe(1+) center having D > 0 and E/D ~ 0.25. This transient species is stable in a glycerol-water glass only up to ~170 K. A combination of electron paramagnetic resonance and Mssbauer spectroscopies provides a powerful means of identifying a transient high-spin Fe(1+) site in a protein scaffold.

published proceedings

  • Inorg Chem

altmetric score

  • 1.35

author list (cited authors)

  • Davydov, R. M., McLaughlin, M. P., Bill, E., Hoffman, B. M., & Holland, P. L.

citation count

  • 13

complete list of authors

  • Davydov, Roman M||McLaughlin, Matthew P||Bill, Eckhard||Hoffman, Brian M||Holland, Patrick L

publication date

  • July 2013