Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: global transcriptomic and proteomic analyses. Academic Article uri icon

abstract

  • Dehalococcoides ethenogenes strain 195 (DE195) was grown in a sustainable syntrophic association with Desulfovibrio vulgaris Hildenborough (DVH) as a co-culture, as well as with DVH and the hydrogenotrophic methanogen Methanobacterium congolense (MC) as a tri-culture using lactate as the sole energy and carbon source. In the co- and tri-cultures, maximum dechlorination rates of DE195 were enhanced by approximately three times (11.00.01 mol per day for the co-culture and 10.10.3 mol per day for the tri-culture) compared with DE195 grown alone (3.80.1 mol per day). Cell yield of DE195 was enhanced in the co-culture (9.00.5 10(7) cells per mol Cl(-) released, compared with 6.80.9 10(7) cells per mol Cl(-) released for the pure culture), whereas no further enhancement was observed in the tri-culture (7.31.8 10(7) cells per mol Cl(-) released). The transcriptome of DE195 grown in the co-culture was analyzed using a whole-genome microarray targeting DE195, which detected 102 significantly up- or down-regulated genes compared with DE195 grown in isolation, whereas no significant transcriptomic difference was observed between co- and tri-cultures. Proteomic analysis showed that 120 proteins were differentially expressed in the co-culture compared with DE195 grown in isolation. Physiological, transcriptomic and proteomic results indicate that the robust growth of DE195 in co- and tri-cultures is because of the advantages associated with the capabilities of DVH to ferment lactate to provide H(2) and acetate for growth, along with potential benefits from proton translocation, cobalamin-salvaging and amino acid biosynthesis, whereas MC in the tri-culture provided no significant additional benefits beyond those of DVH.

published proceedings

  • ISME J

author list (cited authors)

  • Men, Y., Feil, H., Verberkmoes, N. C., Shah, M. B., Johnson, D. R., Lee, P., ... Alvarez-Cohen, L.

citation count

  • 104

complete list of authors

  • Men, Yujie||Feil, Helene||Verberkmoes, Nathan C||Shah, Manesh B||Johnson, David R||Lee, Patrick KH||West, Kimberlee A||Zinder, Stephen H||Andersen, Gary L||Alvarez-Cohen, Lisa

publication date

  • February 2012