In situ detection of live-to-dead bacteria ratio after inactivation by means of synchronous fluorescence and PCA. Academic Article

abstract

• The determination of live and dead bacteria is of considerable significance for preventing health care-associated infection in hospitals, field clinics, and other areas. In this study, the viable (live) and nonviable (dead) bacteria in a sample were determined by means of their fluorescence spectra and principal component analysis (PCA). Data obtained in this study show that it is possible to identify bacteria strains and determine the live/dead ratio after UV light inactivation and antibiotic treatment, in situ, within minutes. In addition, synchronous fluorescence scans enable the identification of bacterial components such as tryptophan, tyrosine, and DNA. Compared with the time-consuming plating and culturing methods, this study renders a means for rapid detection and determination of live and dead bacteria.

authors

• King, Maria
• Rentzepis, Peter

published proceedings

• Proc Natl Acad Sci U S A

altmetric score

• 1

author list (cited authors)

• Li, R., Goswami, U., King, M., Chen, J., Cesario, T. C., & Rentzepis, P. M.

citation count

• 26

complete list of authors

• Li, Runze||Goswami, Umang||King, Maria||Chen, Jie||Cesario, Thomas C||Rentzepis, Peter M

publication date

• January 2018

publisher

• Proceedings of the National Academy of Sciences  Publisher

published in

• Proceedings of the National Academy of Sciences of the United States of America  Journal

keywords

• Bacteria
• Bacteria Inactivation
• Cell Count
• FLUORESCENCE
• Fluorescence Spectroscopy
• Microbial Viability
• Pca
• Principal Component Analysis
• Spectrometry, Fluorescence
• Ultraviolet Therapy

PubMed Central ID

• 29311322

Digital Object Identifier (DOI)

• 10.1073/pnas.1716514115

start page

• 668

end page

• 673

volume

• 115

issue

• 4

URL

• http://dx.doi.org/10.1073/pnas.1716514115