Retinoic acid-induced expression of tissue transglutaminase in human promyelocytic leukemia (HL-60) cells. Academic Article

abstract

• Addition of retinoic acid to human promyelocytic leukemia cells results in a dramatic increase in cellular transglutaminase activity. This increase is due to the induction of a specific intracellular transglutaminase, tissue transglutaminase. Retinoic acid-induced expression of tissue transglutaminase is potentiated by analogues of cyclic AMP. The induction of the enzyme can be detected within 6 h of the addition of the retinoid to the cell and results in increases of the enzyme of at least 50-fold. The induction of HL-60 transglutaminase is a specific response of the cells to retinoic acid and is not seen with other agents that induce HL-60 differentiation. We believe that the induction of tissue transglutaminase is a useful index of the early events in retinoid-regulated gene expression in both normal and transformed cells.

authors

• Davies, Peter

published proceedings

• J Biol Chem

altmetric score

• 3

author list (cited authors)

• Davies, P. J., Murtaugh, M. P., Moore, W. T., Johnson, G. S., & Lucas, D.

citation count

• 150

complete list of authors

• Davies, PJ||Murtaugh, MP||Moore, WT||Johnson, GS||Lucas, D

publication date

• January 1985

publisher

• Elsevier  Publisher

published in

• Journal of Biological Chemistry  Journal

keywords

• Acyltransferases
• Bucladesine
• Cell Line
• Dimethyl Sulfoxide
• Dimethylformamide
• Dose-Response Relationship, Drug
• Enzyme Induction
• Humans
• Hypoxanthine
• Hypoxanthines
• Leukemia, Myeloid, Acute
• Retinol-binding Proteins
• Time Factors
• Transglutaminases
• Tretinoin

Digital Object Identifier (DOI)

• 10.1016/s0021-9258(18)89194-3

start page

• 5166

end page

• 5174

volume

• 260

issue

• 8

URL

• http://dx.doi.org/10.1016/s0021-9258(18)89194-3