Retinoic acid-induced expression of tissue transglutaminase in human promyelocytic leukemia (HL-60) cells. Academic Article uri icon

abstract

  • Addition of retinoic acid to human promyelocytic leukemia cells results in a dramatic increase in cellular transglutaminase activity. This increase is due to the induction of a specific intracellular transglutaminase, tissue transglutaminase. Retinoic acid-induced expression of tissue transglutaminase is potentiated by analogues of cyclic AMP. The induction of the enzyme can be detected within 6 h of the addition of the retinoid to the cell and results in increases of the enzyme of at least 50-fold. The induction of HL-60 transglutaminase is a specific response of the cells to retinoic acid and is not seen with other agents that induce HL-60 differentiation. We believe that the induction of tissue transglutaminase is a useful index of the early events in retinoid-regulated gene expression in both normal and transformed cells.

altmetric score

  • 3

author list (cited authors)

  • Davies, P. J., Murtaugh, M. P., Moore, W. T., Johnson, G. S., & Lucas, D.

citation count

  • 140

publication date

  • April 1985