Phosphorylation of filamin and other proteins in cultured fibroblasts. Academic Article

abstract

• Incubation of subcellular fractions of fibroblasts with [32P]ATP demonstrated 10 phosphoproteins whose phosphorylation can be increased by cyclic AMP or cyclic AMP-dependent protein kinase. One of these phosphoproteins, MW 240,000, resembles the actin binding protein, filamin, and can be selectively precipitated by antibodies to chicken gizzard filamin. Furthermore chicken gizzard filamin can be phosphorylated by skeletal muscle protein kinase and cyclic AMP stimulates this reaction. 1977.

authors

• Davies, Peter

published proceedings

• Biochem Biophys Res Commun

author list (cited authors)

• Davies, P., Shizuta, Y., Olden, K., Gallo, M., & Pastan, I.

citation count

• 36

complete list of authors

• Davies, P||Shizuta, Y||Olden, K||Gallo, M||Pastan, I

publication date

• January 1977

publisher

• Elsevier  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

keywords

• Cell Line
• Cyclic AMP
• Electrophoresis, Polyacrylamide Gel
• Enzyme Activation
• Fibroblasts
• Kinetics
• Magnesium
• Molecular Weight
• Protein Kinases
• Proteins
• Subcellular Fractions

Digital Object Identifier (DOI)

• 10.1016/0006-291x(77)91408-5

start page

• 300

end page

• 307

volume

• 74

issue

• 1

URL

• http://dx.doi.org/10.1016/0006-291x(77)91408-5