Interferon-gamma requires serum retinoids to promote the expression of tissue transglutaminase in cultured human blood monocytes.

abstract

• The culture of HPBM in serum-containing medium induced a large accumulation of the protein cross-linking enzyme, tissue TGase. Immune IFN enhanced the expression of tissue TGase in cultured monocytes. Enzyme-inducing activity, both in normal and IFN-treated cells, was completely blocked by depleting the serum of the lipid fraction. The readdition of retinol at a physiologic concentration (1 micron) to delipidized serum completely restored the enzyme-inducing activity in cultured monocytes. Thus, serum retinoids seem to play an important regulatory role in the expression of tissue TGase gene in differentiating human monocytes.

authors

• Davies, Peter

published proceedings

• J Immunol

altmetric score

• 3

author list (cited authors)

• Mehta, K., Lopez-Berestein, G., Moore, W. T., & Davies, P. J.

citation count

• 24

complete list of authors

• Mehta, K||Lopez-Berestein, G||Moore, WT||Davies, PJ

publication date

• January 1985

publisher

• The American Association of Immunologists  Publisher

published in

• Journal of Immunology  Journal

keywords

• Acyltransferases
• Cells, Cultured
• Enzyme Induction
• Gene Expression Regulation
• Humans
• Interferon-gamma
• Monocytes
• Retinoids
• Tissue Distribution
• Transglutaminases

Digital Object Identifier (DOI)

• 10.4049/jimmunol.134.4.2053

start page

• 2053

end page

• 2056

volume

• 134

issue

• 4

URL

• http://dx.doi.org/10.4049/jimmunol.134.4.2053