Interferon-gamma requires serum retinoids to promote the expression of tissue transglutaminase in cultured human blood monocytes.
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The culture of HPBM in serum-containing medium induced a large accumulation of the protein cross-linking enzyme, tissue TGase. Immune IFN enhanced the expression of tissue TGase in cultured monocytes. Enzyme-inducing activity, both in normal and IFN-treated cells, was completely blocked by depleting the serum of the lipid fraction. The readdition of retinol at a physiologic concentration (1 micron) to delipidized serum completely restored the enzyme-inducing activity in cultured monocytes. Thus, serum retinoids seem to play an important regulatory role in the expression of tissue TGase gene in differentiating human monocytes.
author list (cited authors)
Mehta, K., Lopez-Berestein, G., Moore, W. T., & Davies, P. J.
complete list of authors
Mehta, K||Lopez-Berestein, G||Moore, WT||Davies, PJ