Interferon-gamma requires serum retinoids to promote the expression of tissue transglutaminase in cultured human blood monocytes. Academic Article uri icon

abstract

  • The culture of HPBM in serum-containing medium induced a large accumulation of the protein cross-linking enzyme, tissue TGase. Immune IFN enhanced the expression of tissue TGase in cultured monocytes. Enzyme-inducing activity, both in normal and IFN-treated cells, was completely blocked by depleting the serum of the lipid fraction. The readdition of retinol at a physiologic concentration (1 micron) to delipidized serum completely restored the enzyme-inducing activity in cultured monocytes. Thus, serum retinoids seem to play an important regulatory role in the expression of tissue TGase gene in differentiating human monocytes.

author list (cited authors)

  • Mehta, K., Lopez-Berestein, G., Moore, W. T., & Davies, P. J.

citation count

  • 22

publication date

  • April 1985