Regulation of the expression of the tissue transglutaminase gene by DNA methylation. Academic Article

abstract

• We have investigated the role of DNA methylation in the regulation of the expression of the human tissue transglutaminase gene. Studies on the methylation of the transglutaminase promoter in normal and neoplastic human cells demonstrated that the promoter is methylated in vivo and hypomethylation of the promoter is correlated with constitutive gene expression. Demethylation of the promoter in vivo by treatment of the cells with 5-azacytidine increased transglutaminase expression and hypermethylation of the promoter in vitro suppressed its activity. These studies suggest that alternations in DNA methylation may be one of the mechanisms regulating the tissue-specific expression of the tissue transglutaminase gene.

authors

• Davies, Peter

published proceedings

• Proc Natl Acad Sci U S A

altmetric score

• 6

author list (cited authors)

• Lu, S., & Davies, P. J.

citation count

• 49

complete list of authors

• Lu, S||Davies, PJ

publication date

• April 1997

publisher

• Proceedings of the National Academy of Sciences  Publisher

published in

• Proceedings of the National Academy of Sciences of the United States of America  Journal

keywords

• Base Sequence
• Cells, Cultured
• DNA Methylation
• Dinucleoside Phosphates
• GTP Phosphohydrolases
• GTP-Binding Proteins
• Gene Expression Regulation
• Genes, Reporter
• Humans
• Molecular Sequence Data
• Promoter Regions, Genetic
• Protein Glutamine Gamma Glutamyltransferase 2
• Tissue Distribution
• Transcription, Genetic
• Transglutaminases

PubMed Central ID

• 9114053

Digital Object Identifier (DOI)

• 10.1073/pnas.94.9.4692

start page

• 4692

end page

• 4697

volume

• 94

issue

• 9

URL

• http://dx.doi.org/10.1073/pnas.94.9.4692