Levels of supramolecular chirality of polyglutamine aggregates revealed by vibrational circular dichroism Academic Article uri icon

abstract

  • Polyglutamine (PolyQ) aggregates are a hallmark of several severe neurodegenerative diseases, expanded CAG-repeat diseases in which inheritance of an expanded polyQ sequence above a pathological threshold is associated with a high risk of disease. Application of vibrational circular dichroism (VCD) reveals that these PolyQ fibril aggregates exhibit a chiral supramolecular organization that is distinct from the supramolecular organization of previously observed amyloid fibrils. PolyQ fibrils grown from monomers with Q repeats 35 and above (Q≥35) exhibit approximately 10-fold enhancement of the same VCD spectrum compared to the already enhanced VCD of fibrils formed from Q repeats 30 and below (Q≤30).

author list (cited authors)

  • Kurouski, D., Kar, K., Wetzel, R., Dukor, R. K., Lednev, I. K., & Nafie, L. A.

citation count

  • 25

publication date

  • June 2013

publisher