[9] Use of steady state kinetic methods to elucidate the kinetic and chemical mechanisms of retroviral proteases Academic Article uri icon

abstract

  • Despite the current plethora of structural data of HIV-1 protease and the availability of potent inhibitors, whose structures are based in part on the presumed mechanism of action of this enzyme, our actual understanding of its chemical mechanism has been until now based largely on the precedents of the mammalian and fungal aspartic proteases and static three-dimensional data. The available steady state kinetic data of the protease, as reviewed here, constitute a first step in a detailed description of the mechanism of the enzyme to complement the structural data.

author list (cited authors)

  • Meek, T. D., Rodriguez, E. J., & Angeles, T. S.

citation count

  • 15

publication date

  • January 1994