A rapid and efficient transformation method for the production of large numbers of transgenic potato plants

abstract

• A procedure is described for the rapid and efficient production of transgenic potato (Solanum tuberosum L.) plants using Agrobacterium-based vectors. The key factors are the genotype and the level of kanamycin sulfate used for selection. Optimized culture conditions easily and routinely permitted the isolation of hundreds of individual regenerates expressing -glucuronidase (GUS) transcriptionally fused to promoters from cauliflower mosaic virus (CaMV 35S) or a class-I patatin gene. 1989.

authors

• Park, William

published proceedings

• Plant Science

author list (cited authors)

• Wenzler, H., Mignery, G., May, G., & Park, W.

citation count

• 76

complete list of authors

• Wenzler, Herman||Mignery, Gregory||May, Gregory||Park, William

publication date

• January 1989

publisher

• Elsevier  Publisher

published in

• Plant Science  Journal

keywords

• Genetics

Digital Object Identifier (DOI)

• 10.1016/0168-9452(89)90103-9

start page

• 79

end page

• 85

volume

• 63

issue

• 1

URL

• http://dx.doi.org/10.1016/0168-9452(89)90103-9