The Dcr2p phosphatase destabilizes Sic1p in Saccharomyces cerevisiae. Academic Article

abstract

• Initiation of cell division is controlled by an irreversible switch. In Saccharomyces cerevisiae degradation of the Sic1p protein, an inhibitor of mitotic cyclin/cyclin-dependent kinase complexes, takes place before initiation of DNA replication, at a point called START. Sic1p is phosphorylated by multiple kinases, which can differentially affect the stability of Sic1p. How phosphorylations that stabilize Sic1p are reversed is unknown. Here we show that the Dcr2p phosphatase functionally and physically interacts with Sic1p. Over-expression of Dcr2p destabilizes Sic1p and leads to phenotypes associated with destabilized Sic1p, such as genome instability. Our results identify a novel factor that affects the stability of Sic1p, possibly contributing to mechanisms that trigger initiation of cell division.

authors

• Polymenis, Michael

published proceedings

• Biochem Biophys Res Commun

author list (cited authors)

• Pathak, R., Blank, H. M., Guo, J., Ellis, S., & Polymenis, M.

citation count

• 9

complete list of authors

• Pathak, Ritu||Blank, Heidi M||Guo, Jinbai||Ellis, Sarah||Polymenis, Michael

publication date

• January 2007

publisher

• Elsevier  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

keywords

• Cell Cycle
• Chromosomal Instability
• Cyclin-dependent Kinase Inhibitor Proteins
• Phenotype
• Phosphoric Monoester Hydrolases
• Saccharomyces Cerevisiae
• Saccharomyces Cerevisiae Proteins

Digital Object Identifier (DOI)

• 10.1016/j.bbrc.2007.07.092

start page

• 700

end page

• 704

volume

• 361

issue

• 3

URL

• http://dx.doi.org/10.1016/j.bbrc.2007.07.092