Dietary NaCl affects bleomycin-induced lung fibrosis in mice Academic Article uri icon

abstract

  • PURPOSE: High levels of NaCl in the diet are associated with both cardiac and renal fibrosis, but whether salt intake affects pulmonary fibrosis has not been examined. AIM OF THE STUDY: To test the hypothesis that salt intake might affect pulmonary fibrosis. MATERIALS AND METHODS: Mice were fed low, normal, or high salt diets for 2¬†weeks, and then treated with oropharyngeal bleomycin to induce pulmonary fibrosis, or oropharyngeal saline as a control. RESULTS: As determined by collagen staining of lung sections, and protein levels and cell numbers in the bronchoalveolar lavage (BAL) fluid at 21¬†days after bleomycin, the high salt diet did not exacerbate bleomycin-induced fibrosis, while the low salt diet attenuated fibrosis. For the bleomycin-treated mice, staining of the post-BAL lung sections indicated that compared to the regular salt diet, high salt increased the number of Ly6c-positive macrophages and decreased the number of CD11c and CD206-positive macrophages and dendritic cells. The low salt diet caused bleomycin-induced leukocyte numbers to be similar to control saline-treated mice, but reduced numbers of CD45/collagen-VI positive fibrocytes. In the saline controls, low dietary salt decreased CD11b and CD11c positive cells in lung sections, and high dietary salt increased fibrocytes. CONCLUSIONS: Together, these data suggest the possibility that a low salt diet might attenuate pulmonary fibrosis.

altmetric score

  • 1

author list (cited authors)

  • Chen, W., Pilling, D., & Gomer, R. H.

citation count

  • 4

publication date

  • November 2017