Translational efficiency of phi X174 lysis gene E is unaffected by upstream translation of the overlapping gene D reading frame. Academic Article uri icon


  • The lysis gene E of bacteriophage phi X174 is entirely embedded in gene D. Expression studies of genes D and E in Escherichia coli minicells and lysis times obtained in the presence or absence of D translation showed that the simultaneous expression of gene D does not affect protein E production. Thus, unlike other overlapping gene pairs, gene E expression is independent from the upstream translation of gene D. lacZ fusion studies and primer extension inhibition analysis (toeprinting) revealed an intrinsically weak E ribosome-binding site, which seems to be the major factor determining the low expression rate of the gene and thus proper scheduling of cell lysis.

published proceedings

  • J Bacteriol

author list (cited authors)

  • Blsi, U., Nam, K., Lubitz, W., & Young, R.

citation count

  • 12

complete list of authors

  • Bläsi, U||Nam, K||Lubitz, W||Young, R

publication date

  • October 1990