Isw2 regulates gene silencing at the ribosomal DNA locus in Saccharomyces cerevisiae. Academic Article

abstract

• Three heterochromatin-like domains have been identified in Saccharomyces cerevisiae that are refractory to transcription by Pol II, the silent mating-type loci, telomeres and the ribosomal DNA. Previous work has shown that chromatin remodelers can regulate silent chromatin. Here, we report the findings of an investigation into the role of ISW2 in transcriptional silencing at the rDNA. We show that the levels of retrotransposition and mRNA from a genetically marked Ty1 element located in the rDNA were increased significantly in isw2Delta cells, while transcript levels from Ty1 elements outside of the rDNA were not increased in cells lacking ISW2. Additionally, we show that Isw2 is not required for silencing at a telomere. Our findings demonstrate that Isw2 is required for transcriptional silencing at the rDNA and emphasize the differences in the regulation of transcriptional silencing at silent loci in S. cerevisiae.

authors

• Bryk, Mary

published proceedings

• Biochem Biophys Res Commun

author list (cited authors)

• Mueller, J. E., Li, C., & Bryk, M.

citation count

• 8

complete list of authors

• Mueller, John E||Li, Chonghua||Bryk, Mary

publication date

• October 2007

publisher

• Elsevier  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

keywords

• Adenosine Triphosphatases
• DNA, Ribosomal
• Gene Deletion
• Gene Expression Regulation, Fungal
• Gene Silencing
• Nucleosomes
• Retroelements
• Saccharomyces Cerevisiae
• Telomere
• Transcription Factors
• Transcription, Genetic

PubMed Central ID

• 17689493

Digital Object Identifier (DOI)

• 10.1016/j.bbrc.2007.07.140

start page

• 1017

end page

• 1021

volume

• 361

issue

• 4

URL

• http://dx.doi.org/10.1016/j.bbrc.2007.07.140