Hand-held synchronous scan spectrometer for in situ and immediate detection of live/dead bacteria ratio. Academic Article

abstract

• The design, construction, and operation of a hand-held synchronously scanned, excitation-emission, double monochromator spectrometer is described. Data show that it is possible to record and display within minutes the fluorescence spectra and ratio of live/dead bacteria in situ. Excitation emission matrix contour plots display clearly bacteria fluorescence spectra before and after UV inactivation, respectively. The separation of the fluorescence band maxima of molecular components, such as tryptophan, tyrosine, and DNA, may be distinguished in the diffused fluorescence spectra of bacteria and mixtures.

authors

• Rentzepis, Peter

published proceedings

• Rev Sci Instrum

author list (cited authors)

• Li, R., Goswami, U., Walck, M., Khan, K., Chen, J., Cesario, T. C., & Rentzepis, P. M.

citation count

• 8

complete list of authors

• Li, Runze||Goswami, Umang||Walck, Matthew||Khan, Kasfia||Chen, Jie||Cesario, Thomas C||Rentzepis, Peter M

publication date

• November 2017

publisher

• AIP Publishing  Publisher

published in

• Review of Scientific Instruments  Journal

keywords

• Bacteria
• DNA
• Mortality
• Spectrometry, Fluorescence
• Tryptophan

PubMed Central ID

• 29195411

Digital Object Identifier (DOI)

• 10.1063/1.4991351

start page

• 114301

end page

• 114301

volume

• 88

issue

• 11

URL

• http://dx.doi.org/10.1063/1.4991351