In-Vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules Chapter uri icon


  • Dissolution dynamic nuclear polarization (D-DNP) is a technique to prepare hyperpolarized nuclear spin states, yielding a signal enhancement of several orders of magnitude for liquid-state NMR. Here, we describe experimental procedures for the application of D-DNP in high-resolution NMR of biochemical compounds, to determine the time evolution of biochemical processes and intermolecular interactions.

author list (cited authors)

  • Kim, Y., Wang, Y., Chen, H., & Hilty, C.

citation count

  • 1

complete list of authors

  • Kim, Yaewon||Wang, Yunyi||Chen, Hsueh-Ying||Hilty, Christian

Book Title

  • Protein NMR

publication date

  • November 2017