In-Vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules. Chapter

abstract

• Dissolution dynamic nuclear polarization (D-DNP) is a technique to prepare hyperpolarized nuclear spin states, yielding a signal enhancement of several orders of magnitude for liquid-state NMR. Here, we describe experimental procedures for the application of D-DNP in high-resolution NMR of biochemical compounds, to determine the time evolution of biochemical processes and intermolecular interactions.

authors

• Hilty, Christian

author list (cited authors)

• Kim, Y., Wang, Y., Chen, H., & Hilty, C.

citation count

• 2

complete list of authors

• Kim, Yaewon||Wang, Yunyi||Chen, Hsueh-Ying||Hilty, Christian

Book Title

• PROTEIN NMR: METHODS AND PROTOCOLS

publication date

• January 2018

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Hyperpolarization
• Kinetics
• Nuclear Magnetic Resonance, Biomolecular
• Real-time Nmr
• Spin Correlations
• Spin Relaxation

PubMed Central ID

• 29151209

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-7386-6_8

International Standard Book Number (ISBN) 13

• 978-1-4939-7385-9

start page

• 155

end page

• 168

volume

• 1688

URL

• http://dx.doi.org/10.1007/978-1-4939-7386-6_8