Construction of a bacterial artificial chromosome (BAC) library for potato molecular cytogenetics research. Academic Article

abstract

• Lack of reliable techniques for chromosome identification is the major obstacle for cytogenetics research in plant species with large numbers of small chromosomes. To promote molecular cytogenetics research of potato (Solanum tuberosum, 2n = 4x = 48) we developed a bacterial artificial chromosome (BAC) library of a diploid potato species S. bulbocastanum. The library consists of 23,808 clones with an average insert size of 155 kb, and represents approximately 3.7 equivalents to the potato genome. The majority of the clones in the BAC library generated distinct signals on specific potato chromosomes using fluorescence in situ hybridization (FISH). The hybridization signals provide excellent cytological markers to tag individual potato chromosomes. We also demonstrated that the BAC clones can be mapped to specific positions on meiotic pachytene chromosomes. The excellent resolution of pachytene FISH can be used to construct a physical map of potato by mapping molecular marker-targeted BAC clones on pachytene chromosomes.

authors

• Song, Junqi

published proceedings

• Genome

altmetric score

• 3

author list (cited authors)

• Song, J., Dong, F., & Jiang, J.

citation count

• 49

complete list of authors

• Song, J||Dong, F||Jiang, J

publication date

• February 2000

publisher

• Canadian Science Publishing  Publisher

published in

• Genome  Journal

keywords

• Chromosome Mapping
• Chromosomes, Bacterial
• Cloning, Molecular
• Cytogenetic Analysis
• DNA, Plant
• Gene Library
• Genetic Markers
• Genetic Vectors
• Genome, Plant
• In Situ Hybridization, Fluorescence
• Meiosis
• Solanum Tuberosum

Digital Object Identifier (DOI)

• 10.1139/gen-43-1-199

start page

• 199

end page

• 204

volume

• 43

issue

• 1

URL

• http://dx.doi.org/10.1139/g99-099