In vivo translation of the triple gene block of potato virus X requires two subgenomic mRNAs. Academic Article uri icon

abstract

  • The 25-kilodalton (25K), 12K, and 8K movement proteins of potato virus X are derived from overlapping open reading frames (ORFs). Using an in vivo complementation assay, we have shown that the 25K protein is expressed from a functionally monocistronic mRNA, whereas the 12K and 8K proteins are from a bicistronic mRNA. Translation of the 8K ORF is by leaky ribosome scanning through the 12K ORF.

author list (cited authors)

  • Verchot, J., Angell, S. M., & Baulcombe, D. C.

citation count

  • 79

publication date

  • October 1998