In vivo translation of the triple gene block of potato virus X requires two subgenomic mRNAs.
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The 25-kilodalton (25K), 12K, and 8K movement proteins of potato virus X are derived from overlapping open reading frames (ORFs). Using an in vivo complementation assay, we have shown that the 25K protein is expressed from a functionally monocistronic mRNA, whereas the 12K and 8K proteins are from a bicistronic mRNA. Translation of the 8K ORF is by leaky ribosome scanning through the 12K ORF.
author list (cited authors)
Verchot, J., Angell, S. M., & Baulcombe, D. C.