Structures of Q virions, virus-like particles, and the Q-MurA complex reveal internal coat proteins and the mechanism of host lysis. Academic Article uri icon


  • In single-stranded RNA bacteriophages (ssRNA phages) a single copy of the maturation protein binds the genomic RNA (gRNA) and is required for attachment of the phage to the host pilus. For the canonical Allolevivirus Q the maturation protein, A2, has an additional role as the lysis protein, by its ability to bind and inhibit MurA, which is involved in peptidoglycan biosynthesis. Here, we determined structures of Q virions, virus-like particles, and the Q-MurA complex using single-particle cryoelectron microscopy, at 4.7-, 3.3-, and 6.1- resolutions, respectively. We identified the outer surface of the -region in A2 as the MurA-binding interface. Moreover, the pattern of MurA mutations that block Q lysis and the conformational changes of MurA that facilitate A2 binding were found to be due to the intimate fit between A2 and the region encompassing the closed catalytic cleft of substrate-liganded MurA. Additionally, by comparing the Q virion with Q virus-like particles that lack a maturation protein, we observed a structural rearrangement in the capsid coat proteins that is required to package the viral gRNA in its dominant conformation. Unexpectedly, we found a coat protein dimer sequestered in the interior of the virion. This coat protein dimer binds to the gRNA and interacts with the buried -region of A2, suggesting that it is sequestered during the early stage of capsid formation to promote the gRNA condensation required for genome packaging. These internalized coat proteins are the most asymmetrically arranged major capsid proteins yet observed in virus structures.

published proceedings

  • Proc Natl Acad Sci U S A

altmetric score

  • 21.508

author list (cited authors)

  • Cui, Z., Gorzelnik, K. V., Chang, J., Langlais, C., Jakana, J., Young, R. y., & Zhang, J.

citation count

  • 34

complete list of authors

  • Cui, Zhicheng||Gorzelnik, Karl V||Chang, Jeng-Yih||Langlais, Carrie||Jakana, Joanita||Young, Ry||Zhang, Junjie

publication date

  • October 2017