Analysis of polypeptides synthesized in bovine respiratory syncytial virus-infected cells. Academic Article uri icon

abstract

  • Ten virus-specific polypeptides ranging in molecular weight from approximately 200k to 11k were identified in bovine respiratory syncytial virus (BRSV-)infected cells. Time course analysis of the induction of the viral polypeptides indicated that they could be detected as early as 30 min post-infection and their synthesis reached a plateau 12 h after infection. Cell free translation of total infected-cell mRNA in a rabbit reticulocyte system yielded 7 proteins corresponding in size to virus-specific proteins synthesized in BRSV-infected cells. The P protein was highly phosphorylated; G and F were identified as glycoproteins by [3H]glucosamine labeling. Glycosylation of G protein was largely resistant to tunicamycin, suggesting that the majority of the carbohydrate residues are attached via O-glycosidic bonds, whereas the F protein was N-linked glycosylated. Tunicamycin caused a drastic reduction in the yield of infectious virus titer indicating that the carbohydrate moieties serve a critical role in the infectious cycle of BRSV.

published proceedings

  • Arch Virol

altmetric score

  • 3

author list (cited authors)

  • Mallipeddi, S. K., Samal, S. K., & Mohanty, S. B

citation count

  • 23

complete list of authors

  • Mallipeddi, SK||Samal, SK||Mohanty, SB

publication date

  • March 1990