Identification and structural analysis of a MDV gene encoding a protein kinase. Conference Paper

abstract

• DNA sequence analysis of the BamHI-C fragment of Marek's Disease Virus (MDV) reveals the presence of a 513 amino acid open reading frame (ORF). This ORF codes for a protein with an estimated M(r) of 58,901. Comparison of the amino acid sequence with those available in the Swiss-Prot database indicates extensive homology with a protein kinase (PK) of herpes simplex virus (HSV) and varicella-zoster virus (VZV). In Northern blot hybridization, a transcript of 2.0 kb was detected in MDV (GA strain) infected duck embryo fibroblasts (DEFs). A portion of the ORF was expressed in Escherichia coli as a trpE-fusion protein and used to generate antiserum in New Zealand rabbits. This antiserum specifically detected a protein of 60 kDa in MDV serotype 1, 2 and 3 infected DEFs or chicken embryo fibroblasts (CEFs) by Western blot analysis. This ORF codes for a functional PK.

authors

• Reddy, Sanjay

published proceedings

• Acta Virol

author list (cited authors)

• Reddy, S. M., Sui, D., Wu, P., & Lee, L.

citation count

• 4

complete list of authors

• Reddy, SM||Sui, D||Wu, P||Lee, L

publication date

• April 1999

published in

• Acta Virologica: international journal  Journal

keywords

• Amino Acid Sequence
• Animals
• Blotting, Northern
• Blotting, Western
• Catalytic Domain
• Cells, Cultured
• Chick Embryo
• Genes, Viral
• Herpesvirus 2, Gallid
• Molecular Sequence Data
• Phylogeny
• Protein Kinases
• Rabbits
• Sequence Analysis, DNA
• Sequence Analysis, Protein
• Transcription, Genetic

PubMed Central ID

• 10696441

start page

• 174

end page

• 180

volume

• 43

issue

• 2-3