Trypanosoma lewisi: characterization of complement-activating components. Academic Article

abstract

• At least two complement-activating materials are present in Trypanosoma lewisi preparations. One, a relatively minor component, is trichloroacetic acid (TCA) precipitable and can be inactivated with phenyl methylsufonyl fluoride (PMSF), a broad specificity enzyme inactivator. This substance is therefore probably protin in nature and has enzyme activity, which can activate complement. The second complement-activating material was shown to be stable in TCA and not inhibited by PMSF. This component was shown to contain hexoses and probably some amino acids, although the ultraviolet absorbance was minimal at 280 and 260 nm. Terminal absorbance occurred at 214 nm. The amount of hexoses in different samples corresponded well with their complement-activating capacities. 1977.

authors

• Tizard, Ian

published proceedings

• Exp Parasitol

author list (cited authors)

• Nielsen, K., Sheppard, J., Tizard, I., & Holmes, W.

citation count

• 12

complete list of authors

• Nielsen, K||Sheppard, J||Tizard, I||Holmes, W

publication date

• October 1977

publisher

• Elsevier  Publisher

published in

• Experimental Parasitology  Journal

keywords

• Animals
• Cattle
• Complement System Proteins
• Hexoses
• Pentoses
• Phenylmethylsulfonyl Fluoride
• Proteins
• Subcellular Fractions
• Trypanosoma Lewisi

PubMed Central ID

• 330186

Digital Object Identifier (DOI)

• 10.1016/0014-4894(77)90018-2

start page

• 153

end page

• 160

volume

• 43

issue

• 1

URL

• http://dx.doi.org/10.1016/0014-4894(77)90018-2