Enzyme-linked immunosorbent assay for canine serum ferritin, using monoclonal anti-canine ferritin immunoglobulin G. Academic Article

abstract

• Immunoassay of serum ferritin is currently used to evaluate the clinical iron status of human beings, horses, cattle, and swine. Because ferritins are immunologically species specific, a separate assay must be developed for each species. We have developed an ELISA for serum ferritin in dogs, using a monoclonal anti-canine ferritin antibody. Ferritin standards were linear (r = 0.997) from 0 to 80 ng/ml. Recovery of ferritin from canine serum was 94%. Dilutions of pooled canine serum were linear from 0 to 50% (r = 0.994). Within-assay coefficient of variability was 5.5%, whereas assay-to-assay coefficient of variability ranged from 12.5 to 21%. This assay should provide a nonsurgical means of accurately estimating dogs' iron stores.

authors

• Weeks, Bradley

published proceedings

• Am J Vet Res

author list (cited authors)

• Weeks, B. R., Smith, J. E., & Phillips, R. M.

citation count

• 12

complete list of authors

• Weeks, BR||Smith, JE||Phillips, RM

publication date

• July 1988

published in

• American Journal of Veterinary Research  Journal

keywords

• Animals
• Antibodies, Monoclonal
• Dogs
• Enzyme-Linked Immunosorbent Assay
• Ferritins
• Immunoglobulin G
• Species Specificity

PubMed Central ID

• 3421542

start page

• 1193

end page

• 1195

volume

• 49

issue

• 7