Detergent-free mass spectrometry of membrane protein complexes. Academic Article

abstract

• We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.

authors

• Laganowsky, Arthur

published proceedings

• Nat Methods

altmetric score

• 12.85

author list (cited authors)

• Hopper, J., Yu, Y., Li, D., Raymond, A., Bostock, M., Liko, I., ... Robinson, C. V.

citation count

• 131

complete list of authors

• Hopper, Jonathan TS||Yu, Yvonne Ting-Chun||Li, Dianfan||Raymond, Alison||Bostock, Mark||Liko, Idlir||Mikhailov, Victor||Laganowsky, Arthur||Benesch, Justin LP||Caffrey, Martin||Nietlispach, Daniel||Robinson, Carol V

publication date

• January 2013

publisher

• Springer Nature  Publisher

published in

• Nature Methods  Journal

keywords

• Detergents
• Diacylglycerol Kinase
• Diffusion
• Escherichia Coli
• Escherichia Coli Proteins
• Green Fluorescent Proteins
• Halobacteriaceae
• Lipids
• Magnetic Resonance Spectroscopy
• Mass Spectrometry
• Membrane Proteins
• Micelles
• Microscopy, Electron, Transmission
• Monosaccharide Transport Proteins
• Nanoparticles
• Plasmids
• Sensory Rhodopsins
• Spectrometry, Mass, Electrospray Ionization
• Symporters

Digital Object Identifier (DOI)

• 10.1038/nmeth.2691

start page

• 1206

end page

• 1208

volume

• 10

issue

• 12