Detergent-free mass spectrometry of membrane protein complexes. Academic Article uri icon


  • We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.

published proceedings

  • Nat Methods

altmetric score

  • 12.85

author list (cited authors)

  • Hopper, J., Yu, Y., Li, D., Raymond, A., Bostock, M., Liko, I., ... Robinson, C. V.

citation count

  • 131

complete list of authors

  • Hopper, Jonathan TS||Yu, Yvonne Ting-Chun||Li, Dianfan||Raymond, Alison||Bostock, Mark||Liko, Idlir||Mikhailov, Victor||Laganowsky, Arthur||Benesch, Justin LP||Caffrey, Martin||Nietlispach, Daniel||Robinson, Carol V

publication date

  • January 2013