Picosecond resonance Raman spectrum of the oxyhemoglobin photoproduct. Evidence for an electronically excited state
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The resonance Raman (RR) spectrum of the HbO2 photoproduct has been obtained with 30-ps, 532-nm pulses from a 0.1-mJ/pulse mode-locked Nd:YAG laser. This spectrum differs appreciably from those reported previously for HbCO, and for HbO2 with longer, weaker pulses. These differences are attributed to a prompt photoproduct, which can be associated with a previously observed HbO2 spectral intermediate that decays within 90 ps. The prompt intermediate is inferred, on the basis of the RR band frequencies, to be an electronically excited state of deoxyHb with substantial π-π* character. It might be a triplet π-π* state, formed via the dissociation of triplet O2 from HbO2. © 1982 American Chemical Society.
author list (cited authors)
Terner, J., Voss, D. F., Paddock, C., Miles, R. B., & Spiro, T. G.