Construction of a bovine genomic library of large yeast artificial chromosome clones.

abstract

• We have constructed a yeast artificial chromosome (YAC) library derived from bull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8 x 12 positions) and in 2 x 224 96-well microplates maintained as permanent frozen stocks. An average insert size of 750 kb was estimated from the analysis of 200 randomly selected YACs, giving a sixfold coverage of the bovine genome for the total library. A screening strategy based on the polymerase chain reaction that allows the identification of individual clones has been devised. Screening of the library with six locus-specific markers of interest led to the isolation of 34 positive clones. The proportion of chimeric YACs was estimated from the analysis of a subset of the positive clones: 3 of 9 YACs were found to contain noncontiguous DNA.

published proceedings

• Genomics

altmetric score

• 6

author list (cited authors)

• Libert, F., Lefort, A., Okimoto, R., Womack, J., & Georges, M.

citation count

• 47

complete list of authors

• Libert, F||Lefort, A||Okimoto, R||Womack, J||Georges, M

publication date

• November 1993

publisher

• Elsevier  Publisher

published in

• Genomics  Journal

keywords

• Animals
• Cattle
• Cells, Cultured
• Chromosome Mapping
• Chromosomes, Artificial, Yeast
• Cloning, Molecular
• Gene Library
• Male

PubMed Central ID

• 8288229

Digital Object Identifier (DOI)

• 10.1006/geno.1993.1465

start page

• 270

end page

• 276

volume

• 18

issue

• 2

URL

• http://dx.doi.org/10.1006/geno.1993.1465