Genetic variation of an intestinal leucine arylaminopeptidase (Lap-1) in the mouse and its location on chromosome 9. Academic Article

abstract

• Electrophoretic variation for an intestinal enzyme that cleaves L-leucyl-beta-naphthylamide has been discovered among inbred mouse strains. Several strains including related strains C57BL/6J, C57BL/10J, C57BR/cdJ, C57L/J, and C58/J demonstrate an electrophoretic band of this enzyme that is absent in other strains and stocks thus far observed. The enzyme is tentatively being called leucine arylaminopeptidase (LAP) and the variant genetic locus Lap-1. The presence of the band is determined by an allele designated Lap-1a. Homozygotes for the alternate allele, Lap-1b, are without the band and heterozygotes are, under our electrophoretic conditions, indistinguishable from Lap-1a homozygotes. Data from recombinant inbred lines and a B6D2F1 X DBA/2J backcross established linkage of Lap-1 to dilute (d) and supernatant malic enzyme (Mod-1) on chromosome 9 in the following order: Lap-1-d-Mod-1. The Lap-1 to d map distance was estimated to be 21.3 +/- 4.6 cM from backcross data and 8.1 +/- 4.8 cM from recombinant inbred lines.

authors

• Womack, James

published proceedings

• Biochem Genet

author list (cited authors)

• Womack, J. E., Lynes, M. A., & Taylor, B. A.

citation count

• 36

complete list of authors

• Womack, JE||Lynes, MA||Taylor, BA

publication date

• October 1975

publisher

• Springer Nature  Publisher

published in

• Biochemical Genetics  Journal

keywords

• Alleles
• Animals
• Chromosome Mapping
• Female
• Genes
• Genetic Linkage
• Genotype
• Intestine, Small
• Kidney
• Leucyl Aminopeptidase
• Male
• Mice
• Mice, Inbred Strains
• Recombination, Genetic

Digital Object Identifier (DOI)

• 10.1007/BF00484910

start page

• 511

end page

• 518

volume

• 13

issue

• 9-10

URL

• http://dx.doi.org/10.1007/bf00484910