Phospholipid flippases DnfA and DnfB exhibit differential dynamics within the A. nidulans Spitzenkrper.
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The Spitzenkrper is a structure at the apex of growing cells in many filamentous fungi. Ultrastructural studies indicate that the Spitzenkrper is an organized mass of secretory vesicles, with different types of vesicles present in outer and inner layers. Here, we used live-cell imaging to demonstrate that the phospholipid flippases DnfA and DnfB, which preferentially localize to the outer and inner layers, respectively, exhibit different dynamics in the Spitzenkrper of Aspergillus nidulans. Additionally, deletion of dnfA partially destabilized the Spitzenkrper, while the depletion of cdc50, an essential -subunit of most flippases, had dramatic effects on hyphal tip organization and morphology.
author list (cited authors)
Schultzhaus, Z., Zheng, W., Wang, Z., Mourio-Prez, R., & Shaw, B.
complete list of authors
Schultzhaus, Zachary||Zheng, Wenhui||Wang, Zonghua||Mouriño-Pérez, Rosa||Shaw, Brian