Effects of Exogenous Bone Morphogenetic Protein 2 on the Formation of Mouse Molar Tooth Germ Academic Article uri icon


  • The effects of exogenous bone morphogenetic protein 2 (BMP2) in the formation of mouse molar tooth germ were investigated using an organ culture system of mandibles. Mandibles dissected from mouse embryos at E11 were cultured in BGJb medium supplemented with and without 4 g/ml of BMP2 for 8 days. The mRNA expression of glyceraldehyde-phosphate dehydrogenase (GAPDH), a housekeeping gene, in the mandibles was examined by competitive polymerase chain reaction in combination with reverse-transcription. Cryosections of the mandibles were prepared and stained with hematoxylin and eosin, and the maximum area of molar tooth germ on the section was measured. The distribution of proliferating cell nuclear antigen (PCNA) was analyzed using immunohistochemistry. Exogenous BMP2 did not induce a significant difference in the amount of mRNA for the GAPDH contained in the mandibles. The maximum area of BMP2-treated molar tooth germ was 0.0348 0.0027 mm2 (mean SD, n = 4), which was 78% greater than that of the control molar tooth germ (0.0195 0.0046 mm2) (p<0.01). An enamel knot, an area that lacked immunostaining for PCNA, was found in both the BMP2-treated and control molar tooth germs, but the contour of the enamel knot in the BMP-treated tooth germ appeared to be clearer than that in the control molar tooth germ. These results indicate that exogenous BMP2 increases the size and promotes the formation of molar tooth germ. 2004, Japanese Association for Oral Biology. All rights reserved.

published proceedings

  • Journal of Oral Biosciences

author list (cited authors)

  • Fukui, T., Akutsu, S., Hirashita, A., Oida, S., Diekwisch, T., & Yamane, A.

citation count

  • 0

complete list of authors

  • Fukui, Tadayoshi||Akutsu, Satonari||Hirashita, Ayao||Oida, Shin-Ichiro||Diekwisch, Thomas GH||Yamane, Akira

publication date

  • January 2005