Evidence for control of nitrogen metabolism by a START-dependent mechanism in Saccharomyces cerevisiae.

abstract

• It is generally thought that cell growth and metabolism regulate cell division and not vice versa. Here, we examined Saccharomyces cerevisiae cells growing under conditions of continuous culture in a chemostat. We found that loss of G1 cyclins, or inactivation of the cyclin-dependent kinase Cdc28p, reduced the activity of glutamate synthase (Glt1p), a key enzyme in nitrogen assimilation. We also present evidence indicating that the G1 cyclin-dependent control of Glt1p may involve Jem1p, a DnaJ-type chaperone. Our results suggest that completion of START may be linked to nitrogen metabolism.

authors

• Polymenis, Michael

published proceedings

• Mol Genet Genomics

author list (cited authors)

• Bryan, B. A., McGrew, E., Lu, Y., & Polymenis, M.

citation count

• 5

complete list of authors

• Bryan, BA||McGrew, E||Lu, Y||Polymenis, M

publication date

• January 2004

publisher

• Springer Nature  Publisher

published in

• Molecular Genetics and Genomics  Journal

keywords

• Base Sequence
• CDC28 Protein Kinase, S Cerevisiae
• Cell Cycle
• Cyclins
• DNA, Fungal
• G1 Phase
• Genes, Fungal
• Glutamate Synthase
• Nitrogen
• Saccharomyces Cerevisiae
• Saccharomyces Cerevisiae Proteins

Digital Object Identifier (DOI)

• 10.1007/s00438-003-0957-5

start page

• 72

end page

• 81

volume

• 271

issue

• 1

URL

• http://dx.doi.org/10.1007/s00438-003-0957-5