DETERMINATION OF SOLUBLE AND INSOLUBLE PENTOSES IN THE PRESENCE OF GLUCOSE
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Numerous reagents have been developed for colorimetric measurement of pentoses, including aniline, L-cysteine, phloroglucinol, and orcinol. The use of orcinol reagent is desirable because it is simple to prepare and the colorimetric reaction is easily employed. The orcinol reacts with furfural - which results from the acid degradation of both soluble and insoluble pentoses - to form a green chromogen. The orcinol also reacts with hydroxymethyl furfural - which results from the acid degradation of hexoses - to form a brown-green chromogen. The difference in color between the pentose and hexose chromogens may be exploited to correct for the hexose interference.