A protein antibiotic in the phage Qbeta virion: diversity in lysis targets. Academic Article uri icon

abstract

  • A(2), a capsid protein of RNA phage Qbeta, is also responsible for host lysis. A(2) blocked synthesis of murein precursors in vivo by inhibiting MurA, the catalyst of the committed step of murein biosynthesis. An A(2)-resistance mutation mapped to an exposed surface near the substrate-binding cleft of MurA. Moreover, purified Qbeta virions inhibited wild-type MurA, but not the mutant MurA, in vitro. Thus, the two small phages characterized for their lysis strategy, Qbeta and the small DNA phage phiX174, effect host lysis by targeting different enzymes in the multistep, universally conserved pathway of cell wall biosynthesis.

published proceedings

  • Science

altmetric score

  • 7.056

author list (cited authors)

  • Bernhardt, T. G., Wang, I. N., Struck, D. K., & Young, R.

citation count

  • 110

complete list of authors

  • Bernhardt, TG||Wang, IN||Struck, DK||Young, R

publication date

  • June 2001