Isolation and purification of closely related citrus limonoid glucosides by flash chromatography. Academic Article

abstract

• Several citrus limonoid glycosides have proved to be particularly difficult to purify using conventional techniques. A reversed-phase flash chromatographic technique has been developed for the separation and isolation of the closely related limonoid glucosides, nomilin 17-beta-D-glucopyranoside and nomilinic acid 17-beta-D-glucopyranoside, with confirmation of their identities by electrospray ionisation mass spectrometry. Furthermore, the semi-purification of the mixture of glucosides enriched with flavanone glucosides such as naringin, narirutin and other limonoid glucosides was obtained. The closely eluting glucosides were successfully separated to achieve a good yield and purity of 93%.

authors

• Patil, Bhimanagouda

published proceedings

• Phytochem Anal

altmetric score

• 3

author list (cited authors)

• Raman, G., Cho, M., Brodbelt, J. S., & Patil, B. S.

citation count

• 25

complete list of authors

• Raman, Girija||Cho, Minhee||Brodbelt, Jennifer S||Patil, Bhimanagouda S

publication date

• May 2005

publisher

• Wiley  Publisher

published in

• Phytochemical Analysis  Journal

keywords

• Chromatography
• Citrus Paradisi
• Glucosides
• Limonins
• Seeds
• Spectrometry, Mass, Electrospray Ionization

PubMed Central ID

• 15997847

Digital Object Identifier (DOI)

• 10.1002/pca.835

start page

• 155

end page

• 160

volume

• 16

issue

• 3

URL

• http://dx.doi.org/10.1002/pca.835