Quantification of Health-Promoting Compounds by Quantitative 1 HNMR Spectroscopy

© 2014 American Chemical Society. Nuclear magnetic resonance (NMR) spectroscopy is a wellknown analytical technique for simultaneous quantitation and structural elucidation of small molecules and macromolecules. In natural products research, quantitative NMR (qNMR) gives valuable information on metabolites. qNMR is robust with high precision, good reproducibility and is non-destructive, compared to traditional analytical methods. It has proven to be rapid, highly reliable for the determination of purity, examination of impurities, metabolic profiling, and for the quantitation of single entities in complex mixtures without fractionation or isolation. Therefore, qNMR can be used to simultaneously identify and quantify multiple metabolites. This chapter discusses current challenges in quantitation, metabolomics, and sample preparation, as well as the selection of references for quantitation of health-promoting compounds.

Quantification of Health-Promoting Compounds by Quantitative 1 HNMR Spectroscopy Conference Paper