Production and enzyme activity of an extracellular amylase from Aspergillus niger, Aspergillus oryzae and Bacillus subtilis
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The study of amylase production were carried out with Bacillus subtilis bacterial and Aspergillus niger and Aspergillus oryzae fungal organism. The Bacillus were cultured in a mineral medium containing yeast extract, peptone, MgSO4, NaCl, CaCl2 and Aspergillus were cultured in a mineral medium incorporated with glucose, glycerol and yeast extract. The minerals in media shortened the lag phase greatly and improved the growth and amylase synthesis. The optimum temperature and pH for activity were 40°C and 7.0 respectively. The addition of substrate concentration at 1% exhibits maximum amylase activity. © Global Science Publications.
author list (cited authors)
Aglave, B. A., Lele, P. P., Lokhande, M. O., & Pagare, R. S.