RFLPs in mitochondrial and nuclear DNA indicate low levels of genetic diversity in the oak wilt pathogen Ceratocystis fagacearum.

abstract

• Genetic diversity in the oak wilt pathogen Ceratocystis fagacearum was assessed using restriction fragment length polymorphisms (RFLPs) of the mitochondrial DNA (mtDNA) and anonymous RFLP loci in the nuclear DNA (nuDNA). No genetic variation was detected in the mtDNA among 27 isolates sampled from a broad geographical area. Southern hybridization to 100 anonymous, random, nuDNA probes detected a low level of variation among nine of the isolates. Only 35 out of 437 probe-enzyme combinations detected RFLPs. Most of the RFLPs appeared to result from insertions and deletions of less than 200 bp. A composite multilocus haplotype based on hybridization to six anonymous probes could differentiate each of the nine isolates tested, suggesting that these probes may be useful for further studies of the population biology and epidemiology of this pathogen. Hypotheses are presented to account for the low level of genetic variation.

authors

• Appel, David

published proceedings

• Curr Genet

author list (cited authors)

• Kurdyla, T. M., Guthrie, P. A., McDonald, B. A., & Appel, D. N.

citation count

• 19

complete list of authors

• Kurdyla, TM||Guthrie, PA||McDonald, BA||Appel, DN

publication date

• March 1995

publisher

• Springer Nature  Publisher

published in

• Current Genetics  Journal

keywords

• Ascomycota
• DNA, Fungal
• DNA, Mitochondrial
• Genetic Markers
• Genetic Variation
• Haplotypes
• Polymorphism, Restriction Fragment Length

Digital Object Identifier (DOI)

• 10.1007/BF00352107

start page

• 373

end page

• 378

volume

• 27

issue

• 4

URL

• http://dx.doi.org/10.1007/bf00352107