Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana. Academic Article uri icon

abstract

  • A bacterial artificial chromosome (BAC) library from the dihaploid melon line 'PIT92' was constructed with a 6 times coverage of the haploid melon genome. A contig of four BACs around the MRGH63 resistance gene homologue fragment was created. The complete sequence of a 117-kb BAC clone allowed to determine two clearly defined regions, the first one containing a cluster of three resistance gene homologues. Separated by a retrotransposon, that contains large long terminal repeats, the second region presents a group of genes with a conserved distribution in two regions of the Arabidopsis genome. The detailed analysis of this region provides a description of the gene structure and the presence of repetitive sequences in a defined fragment of the genome of Cucumis melo.

published proceedings

  • Plant Mol Biol

author list (cited authors)

  • van Leeuwen, H., Monfort, A., Zhang, H., & Puigdomnech, P.

citation count

  • 49

complete list of authors

  • van Leeuwen, Hans||Monfort, Amparo||Zhang, Hong-Bin||Puigdomènech, Pere

publication date

  • January 1, 2003 11:11 AM