A simple method for isolation of megabase DNA from cotton Academic Article uri icon

abstract

  • A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning. 1994 Kluwer Academic Publishers.

published proceedings

  • Plant Molecular Biology Reporter

author list (cited authors)

  • Zhao, X., Zhang, H., Wing, R. A., & Paterson, A. H.

citation count

  • 5

complete list of authors

  • Zhao, Xinping||Zhang, Hong-Bin||Wing, Rod A||Paterson, Andrew H

publication date

  • January 1, 1994 11:11 AM