A simple method for isolation of megabase DNA from cotton Academic Article

abstract

• A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning. 1994 Kluwer Academic Publishers.

authors

• Zhang, Hongbin

published proceedings

• Plant Molecular Biology Reporter

author list (cited authors)

• Zhao, X., Zhang, H., Wing, R. A., & Paterson, A. H.

citation count

• 5

complete list of authors

• Zhao, Xinping||Zhang, Hong-Bin||Wing, Rod A||Paterson, Andrew H

publication date

• January 1994

publisher

• Springer Nature  Publisher

published in

• Plant Molecular Biology Reporter  Journal

keywords

• Biotechnology
• Genetics

Digital Object Identifier (DOI)

• 10.1007/bf02668372

start page

• 110

end page

• 115

volume

• 12

issue

• 2

URL

• http://dx.doi.org/10.1007/bf02668372