A bac library constructed to the rice cultivar "Minghui 63" for cloning genes of agronomic importance

"Minghui 63" is the restorer line for a number of the most important commercial rice hybrids varieties in China. To facilitate long-term commitment in genetic analysis and molecular cloning of the superior genes in the genome of "Minghui 63", the authors have constructed a large-insert genomic DNA library using the bacterial artificial chromosome (BAC) cloning vector (pBe-loBAC11). Size fractionated Hin d III digest of genomic DNA was ligated to the BAC vector, and the ligation mixture was used to transform the bacterial strain DH10B. A total of over 26 000 clones were obtained with the average insert size of about 150 kb, ranging from 90 to 240 kb. These clones thus represent 9 x rice haploid genome equivalents. The library is now being used for physical mapping of several genomic regions for map-based gene cloning.

A bac library constructed to the rice cultivar "Minghui 63" for cloning genes of agronomic importance Academic Article