Work activities at complex urban freeway interchanges often result in modified lane assignments and the temporary misalignment of existing overhead guide signs with the travel lanes. Researchers conducted a driving simulator study to determine whether the location and accuracy of driver lane changes made before major freeway-to-freeway interchanges were affected by temporarily misaligned permanent guide signing, temporary guide signing, and route shield pavement markings. The results of this study verify that when the lane assignment arrows on existing overhead guide signs are covered and the signs are temporarily misaligned with the travel lanes, drivers are not clear about which lane they need to be in to reach their destination; thus they wait to make a lane change until they are closer to the exit ramp gore area. Additional path guidance information presented to drivers before the interchange can be beneficial. The results of the study also indicate that smaller temporary lane assignment signs, route shield pavement markings upstream of the interchange, or both encourage drivers to make their lane change further upstream of the exit ramp gore area. Either of these devices or a combination should be used to provide drivers with additional path guidance information in work zones in which the existing overhead guide signs are temporarily misaligned with the travel lanes and the lane assignment arrows are covered.