Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis. Academic Article

abstract

• Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E. faecalis strains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption of orfde4, encoding a putative glycosyl transferase, and orfde6, a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoid E. faecalis isolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen.

authors

• Xu, Yi

published proceedings

• Infect Immun

altmetric score

• 3

author list (cited authors)

• Xu, Y., Singh, K. V., Qin, X., Murray, B. E., & Weinstock, G. M.

citation count

• 88
• 92

complete list of authors

• Xu, Y||Singh, KV||Qin, X||Murray, BE||Weinstock, GM

editor list (cited editors)

• Tuomanen, E. I.

publication date

• February 2000

publisher

• American Society for Microbiology  Publisher

published in

• Infection and Immunity  Journal

keywords

• Animals
• Enterococcus Faecalis
• Genes, Bacterial
• Humans
• Mice
• Multigene Family
• Mutation
• Open Reading Frames
• Peritonitis
• Polysaccharides, Bacterial
• RNA, Messenger
• Reverse Transcriptase Polymerase Chain Reaction
• Transcription, Genetic

PubMed Central ID

• 10639451

Digital Object Identifier (DOI)

• 10.1128/IAI.68.2.815-823.2000

start page

• 815

end page

• 823

volume

• 68

issue

• 2

URL

• http://dx.doi.org/10.1128/iai.68.2.815-823.2000