Parathyroid hormone-related protein purified from a human lung cancer cell line. Academic Article uri icon

abstract

  • A protein with biological activities similar to parathyroid hormone (PTH) has been purified from serum-free culture medium obtained from a human lung cancer cell line (BEN). A major protein band of 18 kDa was obtained on NaDodSO4/polyacrylamide gels, with faint bands at 35 kDa and 67 kDa. Biological activity was associated only with the 18-kDa band. Amino acid sequence analysis of the material purified by HPLC revealed that 8 of the 16 residues were identical with those of human PTH. Antibody raised to a corresponding synthetic peptide recognized the PTH-related material but showed less than 1% cross-reactivity with human PTH amino-terminal peptides. BEN cells contained PTH DNA, but not PTH messenger RNA, indicating involvement of another gene. The purified PTH-related protein had a specific biological activity approximately equal to 6 times greater than that of bovine PTH(1-34). The PTH-related protein may have a role in the syndrome of humoral hypercalcemia of malignancy.

published proceedings

  • Proc Natl Acad Sci U S A

altmetric score

  • 11.2

author list (cited authors)

  • Moseley, J. M., Kubota, M., Diefenbach-Jagger, H., Wettenhall, R. E., Kemp, B. E., Suva, L. J., ... Zajac, J. D.

citation count

  • 628

complete list of authors

  • Moseley, JM||Kubota, M||Diefenbach-Jagger, H||Wettenhall, RE||Kemp, BE||Suva, LJ||Rodda, CP||Ebeling, PR||Hudson, PJ||Zajac, JD

publication date

  • July 1987