Production of parathyroid hormone-related protein by a rat parathyroid cell line. Academic Article uri icon

abstract

  • Parathyroid hormone-related protein (PTHrP), the peptide associated with humoral hypercalcemia of malignancy, has been identified in fetal and adult parathyroid glands. We here report a sub-clone of a rat parathyroid cell line which secretes a single peptide species corresponding in size to PTHrP(1-84). Biological activity of the secretion product was blocked by a specific antiserum against PTHrP, but not by parathyroid hormone (PTH) antiserum. Secretion of PTHrP by these cells was regulated by extracellular calcium in the physiological range. A single messenger RNA species for PTHrP was identified, though PTH mRNA could not be shown in these cells. Hybrid CAT genes containing 700-1000 bp of 5'-flanking DNA from the human PTH or PTHrP genes were transfected into these cells, and the PTHrP gene was expressed at 10-fold higher levels than the PTH gene. These cells thus provide a valuable model system for investigation expression of PTHrP in a non-transformed cell line.

published proceedings

  • Mol Cell Endocrinol

author list (cited authors)

  • Zajac, J. D., Callaghan, J., Eldridge, C., Diefenbach-Jagger, H., Suva, L. J., Hudson, P., ... Pasquini, G.

citation count

  • 27

complete list of authors

  • Zajac, JD||Callaghan, J||Eldridge, C||Diefenbach-Jagger, H||Suva, LJ||Hudson, P||Moseley, JM||Michelangeli, VP||Pasquini, G

publication date

  • November 1989