Characterization of specific immune responses of mice inoculated with recombinant vaccinia virus expressing an 18-kilodalton outer membrane protein of Brucella abortus. uri icon

abstract

  • Using the shuttle vector pMCO2 and the vaccinia virus wild-type WR strain, we constructed a recombinant virus expressing an 18-kDa outer membrane protein of Brucella abortus. BALB/c mice inoculated with this virus produced 18-kDa protein-specific antibodies, mostly of immunoglobulin G2a isotype, and in vitro stimulation of splenocytes from these mice with purified maltose binding protein-18-kDa protein fusion resulted in lymphocyte proliferation and gamma interferon production. However, these mice were not protected against a challenge with the virulent strain B. abortus 2308. Disruption of the 18-kDa protein's gene in vaccine strain B. abortus RB51 did not affect either the strain's protective capabilities or its in vivo attenuation characteristics. These observations suggest that the 18-kDa protein plays no role in protective immunity.

published proceedings

  • Clin Diagn Lab Immunol

altmetric score

  • 3

author list (cited authors)

  • Vemulapalli, R., Cravero, S., Calvert, C. L., Toth, T. E., Sriranganathan, N., Boyle, S. M., Rossetti, O. L., & Schurig, G. G.

citation count

  • 42

complete list of authors

  • Vemulapalli, R||Cravero, S||Calvert, CL||Toth, TE||Sriranganathan, N||Boyle, SM||Rossetti, OL||Schurig, GG

publication date

  • January 2000