Molecular detection of Leptospira kirschneri in tissues of a prematurely born foal.

abstract

• Leptospirosis was identified to be the possible cause of premature birth in a foal on a farm with a history of repeated abortions. Using an appropriate polymerase chain reaction (PCR) assay, the presence of Leptospira kirschneri was detected in the tissues of the prematurely born foal. Further confirmation of L. kirschneri was obtained by nucleotide sequence analysis of the PCR-amplified DNA fragment and the partial 16S ribosomal RNA gene sequence. This report further supports mounting evidence that a PCR assay capable of detecting L. kirschneri should be included in routine diagnostic investigations in which Leptospira spp. infection is suspected.

authors

• Vemulapalli, Ramesh

published proceedings

• J Vet Diagn Invest

author list (cited authors)

• Vemulapalli, R., Langohr, I. M., Sanchez, A., Kiupel, M., Bolin, C. A., Wu, C., & Lin, T.

citation count

• 11

complete list of authors

• Vemulapalli, Ramesh||Langohr, Ingeborg M||Sanchez, Alfredo||Kiupel, Matti||Bolin, Carol A||Wu, Ching-Ching||Lin, Tsang-Long

publication date

• January 2005

publisher

• SAGE Publications  Publisher

published in

• Journal of Veterinary Diagnostic Investigation  Journal

keywords

• Animals
• DNA, Bacterial
• Female
• Horse Diseases
• Horses
• Leptospira
• Leptospirosis
• Polymerase Chain Reaction
• Pregnancy
• Premature Birth
• Species Specificity

PubMed Central ID

• 15690955

Digital Object Identifier (DOI)

• 10.1177/104063870501700114

start page

• 67

end page

• 71

volume

• 17

issue

• 1

URL

• http://dx.doi.org/10.1177/104063870501700114

user-defined tag

• 3 Good Health and Well-Being