A recombinant vaccine expressing a mammalian Mycobacterium sp. antigen is immunostimulatory but not protective in striped bass. Academic Article

abstract

• A recombinant vaccine was constructed for piscine mycobacteriosis utilizing a Brucella abortus strain RB51 vector expressing a mammalian Mycobacterium sp. 85A antigen. Juvenile striped bass were inoculated with the resulting construct at doses equivalent to 10(6), 10(7), 10(8), 10(9), and 10(10) colony-forming units/fish. Blood and tissue samples from these fish demonstrated significant specific humoral and cell-mediated immune responses towards the 85A antigen in a dose-dependent manner. However, survival studies determined that inoculated fish failed to demonstrate cross-protective responses after live Mycobacterium marinum challenge 70 days post-inoculation.

authors

• Vemulapalli, Ramesh

published proceedings

• Vet Immunol Immunopathol

author list (cited authors)

• Pasnik, D. J., Vemulapalli, R., Smith, S. A., & Schurig, G. G.

citation count

• 8

complete list of authors

• Pasnik, David J||Vemulapalli, Ramesh||Smith, Stephen A||Schurig, Gerhardt G

publication date

• January 2003

publisher

• Elsevier  Publisher

published in

• Veterinary Immunology and Immunopathology  Journal

keywords

• Amino Acid Sequence
• Animals
• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Vaccines
• Base Sequence
• Bass
• Enzyme-Linked Immunosorbent Assay
• Fish Diseases
• Immunization
• Molecular Sequence Data
• Mycobacterium
• Mycobacterium Infections
• Recombinant Proteins
• Sequence Alignment
• Survival Analysis
• Vaccines, Synthetic

Digital Object Identifier (DOI)

• 10.1016/s0165-2427(03)00099-0

start page

• 43

end page

• 52

volume

• 95

issue

• 1-2

URL

• http://dx.doi.org/10.1016/s0165-2427(03)00099-0

user-defined tag

• 3 Good Health and Well-Being