Prevalence and molecular detection of Anaplasma marginale, Babesia bovis and Babesia bigemina in cattle from Puntarenas Province, Costa Rica. Academic Article

abstract

• A study was conducted in 2008 to determine the prevalence of Anaplasma and Babesia infections in cattle in the Puntarenas Province of Costa Rica. Blood samples were taken from a total of 449 cattle during the month of March at 30 farms in the region of Espiritu Santu, Costa Rica. Commercially available enzyme-linked immunosorbent assays (ELISA) were used to determine presence of antibodies to Babesia bigemina and Anaplasma marginale, and real-time PCR was used to determine the presence of DNA from the disease-causing organisms. The ELISA results indicated that 87.5% of the cattle sampled were positive for antibodies to A. marginale, while 59.1% were positive for antibodies to B. bigemina. The real-time PCR results showed that 235 cattle were carrying A. marginale DNA (56.9%), 6 with B. bigemina DNA (1.34%), and 2 with B. bovis DNA (0.45%).

authors

• Vemulapalli, Ramesh

published proceedings

• Vet Parasitol

author list (cited authors)

• Shebish, E., Vemulapalli, R., & Oseto, C.

citation count

• 15

complete list of authors

• Shebish, Emily||Vemulapalli, Ramesh||Oseto, Christian

publication date

• January 2012

publisher

• Elsevier  Publisher

published in

• Veterinary Parasitology  Journal

keywords

• Anaplasma Marginale
• Animals
• Babesia
• Babesiosis
• Cattle
• Costa Rica
• Enzyme-Linked Immunosorbent Assay
• Prevalence
• Real-Time Polymerase Chain Reaction

Digital Object Identifier (DOI)

• 10.1016/j.vetpar.2012.03.009

start page

• 164

end page

• 167

volume

• 188

issue

• 1-2

URL

• http://dx.doi.org/10.1016/j.vetpar.2012.03.009