A Method for the Determination of α-Dicarbonyl Compounds1 Academic Article uri icon


  • A method is reported for determining the α-dicarbonyls glyoxal, methylglyoxal, and diacetyl. The carbonyls were reacted at pH 8 with .05% aqueous solution of o-phenylenediamine for 4 h at 25°C to form quinoxalines. The derivatives were extracted with chloroform, transferred to methanol, and separated by HPLC on a Supelcosil LC-18 column with methanol water as the mobile phase. The method was applied to several dairy cultures and cheese varieties. The amounts of glyoxal, methylglyoxal, and diacetyl in the cultures varied from 2 to 227, 0 to 7, and 1 to 11 µg/ml, respectively, depending on the species, strain, and culture medium. © 1989, American Dairy Science Association. All rights reserved.

author list (cited authors)

  • Bednarski, W., Jedrychowski, L., Hammond, E. G., & Nikolov, Z. L.

publication date

  • January 1, 1989 11:11 AM