A METHOD FOR THE DETERMINATION OF ALPHA-DICARBONYL COMPOUNDS Academic Article uri icon

abstract

  • A method is reported for determining the -dicarbonyls glyoxal, methylglyoxal, and diacetyl. The carbonyls were reacted at pH 8 with .05% aqueous solution of o-phenylenediamine for 4 h at 25C to form quinoxalines. The derivatives were extracted with chloroform, transferred to methanol, and separated by HPLC on a Supelcosil LC-18 column with methanol water as the mobile phase. The method was applied to several dairy cultures and cheese varieties. The amounts of glyoxal, methylglyoxal, and diacetyl in the cultures varied from 2 to 227, 0 to 7, and 1 to 11 g/ml, respectively, depending on the species, strain, and culture medium. 1989, American Dairy Science Association. All rights reserved.

published proceedings

  • JOURNAL OF DAIRY SCIENCE

author list (cited authors)

  • BEDNARSKI, W., JEDRYCHOWSKI, L., HAMMOND, E. G., & NIKOLOV, Z. L.

citation count

  • 32

complete list of authors

  • BEDNARSKI, W||JEDRYCHOWSKI, L||HAMMOND, EG||NIKOLOV, ZL

publication date

  • January 1, 1989 11:11 AM