Comparative study of two chemical protocols for creating white spot lesions: An in vitro FluoreCam evaluation
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AIM: The aim of the present study was to compare the effectiveness and practicality of two commonly-used protocols for white spot lesion creation. METHODS: Sound posterior human teeth were sectioned into halves and randomly allocated into two groups (n=20). Using the FluoreCam system, the enamel surfaces were imaged under standardized conditions, and baseline data (area, intensity, and impact) were recorded. Specimens were covered with an acid-resistant polish, leaving a 2×6-mm enamel window. Group 1 was immersed in Queiroz solution (64 hours/37°C/pH5 with agitation); Group 2 was immersed in Buskes solution (14 days/37°C/pH5 with agitation). The specimens then were imaged again. Within-group changes that occurred over time were evaluated using the Wilcoxon signed-rank test. Between-group differences were analyzed using the Mann-Whitney U-test. Polarized light microscopy (PLM) was used to calculate the mean lesion depth of representative specimens. RESULTS: Each protocol showed significant changes in the surface area, intensity and impact of demineralization over time (P<.05). There were no statistically-significant differences (P=.53, P=.2, P=.74) between the two groups. PLM showed that the mean lesion depths of representative specimens were 51.4 and 73.0 μm for groups 1 and 2, respectively. CONCLUSIONS: While both protocols produce similar amounts of demineralization, the Queiroz solution is more practical, as it requires less time.
author list (cited authors)
Abufarwa, M., Noureldin, A., Campbell, P. M., & Buschang, P. H.