NADPH oxidase overexpression in human colon cancers and rat colon tumors induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Academic Article uri icon


  • NADPH oxidase/dual-oxidase (Nox/Duox) family members have been implicated in nuclear factor kappa-B (NFB)-mediated inflammation and inflammation-associated pathologies. We sought to examine, for the first time, the role of Nox/Duox and NFB in rats treated with the cooked meat heterocyclic amine carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). In the PhIP-induced colon tumors obtained after 1 year, Nox1, Nox4, NFB-p50 and NFB-p65 were all highly overexpressed compared with their levels in adjacent normal-looking colonic mucosa. Nox1 and Nox4 mRNA and protein levels also were markedly elevated in a panel of primary human colon cancers, compared with their matched controls. In HT29 human colon cancer cells, Nox1 knockdown induced G1 cell cycle arrest, whereas in Caco-2 cells there was a strong apoptotic response, with increased levels of cleaved caspase-3, -6, -7 and poly(ADP-ribose)polymerase. Nox1 knockdown blocked lipopolysaccharide-induced phosphorylation of IB kinase, inhibited the nuclear translocation of NFB (p50 and p65) proteins, and attenuated NFB DNA binding activity. There was a corresponding reduction in the expression of downstream NFB targets, such as MYC, CCND1 and IL1. The results provide the first evidence for a role of Nox1, Nox4 and NFB in PhIP-induced colon carcinogenesis, including during the early stages before tumor onset. Collectively, the findings from this investigation and others suggest that further work is warranted on the role of Nox/Duox family members and NFB in colon cancer development.

published proceedings

  • Int J Cancer

author list (cited authors)

  • Wang, R., Dashwood, W., Nian, H., Lhr, C. V., Fischer, K. A., Tsuchiya, N., ... Dashwood, R. H.

citation count

  • 53

complete list of authors

  • Wang, Rong||Dashwood, Wan-Mohaiza||Nian, Hui||Löhr, Christiane V||Fischer, Kay A||Tsuchiya, Naoto||Nakagama, Hitoshi||Ashktorab, Hassan||Dashwood, Roderick H

publication date

  • June 2011